Generation of a variety of organs and tissues from human pluripotent stem cells (hPSCs) has been attempted in vitro. We here present a simple and efficient method for induction of hypothalamic and pituitary tissues from hPSCs. On provision of exogenous agents important for early hypothalamus-pituitary organogenesis. including bone morphogenetic protein 4 and activators of sonic hedgehog. https://atlanticknifers.shop/product-category/baton/